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Well if your template is working, the only thing (that I know of at least) that will limit you is your bandwidth and manpower. We do large groups at a time when doing go lives at our sites, and haven't had any issues other than bandwidth.
Hope that answers your question
Thanks for the info, I have seen a post on here before saying there was an issue with the number of PC's that can be picked up by the TFTP Server and I have seen issues myself when trying to PXE boot more than 15 PC's at once.