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I've had a similar experience when one of our admins changed the password of the user that has been used by the Application Pools by accident. But I can imagine, that there might be something more complex behind it.
If you've not done so already:
- Check if the user in your application pool is still active.
- Check if the password expired.
- Check if the password of the user has been changed.
- Check if the user has been removed from local groups (especially the local administrators).
- If using integrated security for DB access, check if the user has been removed from the DB security settings.
- Check if all App Pools are running and if they are stable (sometimes if something is wrong with the user accounts running the app pools, they will simply stop to run without any notice).
- Try and use the built-in account "NetworkService" for a test in your App Pool.
- To work around the "The network path was not found." message, use your IIS Manager to switch the user for your App Pool.
- If possible, run an IISRESET on all affected servers.
I think that should cover the most common problems.
Thank you for your feedback! With further troubleshooting we found the root cause to be that somewhere along the line something happened with the service account. When the app pools were recycled with the previously working service accounts, NTFS permissions were changed on the folder "Program Files (x86)\LANDESK\Service Desk\WebApp\WebAccess\Content" and access was removed for the local server users. Somehow this fundamentally breaks Service desk's ability to pass credentials through with integrated LogOn.
I have verified with out other teams that the account is still active and the password is not expired, but it is still throwing the "Network Path Not Found" error and simultaneously stripping the aforementioned access away from that folder. In the meantime we have switched the App Pool to use "NetworkService" until we isolate the issue further. This is not yet resolved, but we are at least making progress.